I Can Fly is a SimpleFlips meme originating from the Super Mario 64 romhack Shrek 64, in which Mario is replaced by Donkey. Mario's "Yahoo!" sound effect used during long jumps is replaced with a sound byte of Donkey saying "I can fly!"The joke became popular due to its frequency in the Shrek 64 stream. Additionally, as the same stream was still going, AutisticYui gave a song donation of 1, 2 Oatmeal with the lyrics replaced with I Can Fly, somehow managing to create and upload a remix in around 20 minutes.
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